Bovine monoclonal antibodies to the F5 (K99) pilus antigen of E. coli, produced by murine/bovine hybridomas
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منابع مشابه
Using various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (BLV) gp51SU
The objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51SU. The four antigen preparations for immunization ofBALB/c mice were: CL: BLV-FLK cell lysate, UF: a fraction of CL (between 30 and 100 kDa), WVP: wholevirus particles and SP: with ion exchange chromatography, gp51SU was semipurified. A total of ninesucce...
متن کاملDevelopment of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carri...
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Using the human lymphoblastoid cell line, GM 4672, and PBL of Gambian adults immune to Plasmodium falciparum (Pf) malaria, we have produced human-human hybridomas and selected those that produce mAb against Pf antigens. The fusion frequency, using PWM-stimulated donor lymphocytes was between 6.8 X 10(-5) and 1.5 X 10(-6). Using immune fluorescence, immune precipitation, and Pf in vitro growth i...
متن کاملProduction and characterization of monoclonal antibodies to a pilus colonization factor (colonization factor antigen III) of human enterotoxigenic Escherichia coli.
Three monoclonal antibodies (MAbs) to a pilus colonization factor (colonization factor antigen III [CFA/III]) of human enterotoxigenic Escherichia coli (ETEC) were developed and characterized. All of the MAbs isolated belonged to the immunoglobulin G2a subclass. The specificity of these MAbs for CFA/III pili was demonstrated by the immunogold-labeling technique. The presence of more than one ep...
متن کاملusing various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (blv) gp51su
the objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51su. the four antigen preparations for immunization ofbalb/c mice were: cl: blv-flk cell lysate, uf: a fraction of cl (between 30 and 100 kda), wvp: wholevirus particles and sp: with ion exchange chromatography, gp51su was semipurified. a total of ninesucce...
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ژورنال
عنوان ژورنال: Veterinary Immunology and Immunopathology
سال: 1987
ISSN: 0165-2427
DOI: 10.1016/0165-2427(87)90085-7